Summary
Type: SPE
Application #: 305770
Catalog#: 730031 - SPE Cartridges, Chromabond, NH2, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning: 2 mL methanol, then 2 mL chloroform, at last 2 mL n-hexane
(Do not let run the column dry.)
Sample aspiration: lipid extraxt, dissolved in 300 µL chloroform is passed through the column by vacuum
Elution 1: fraction of neutral lipids are eluted with 3 mL chloroform – isopropanol (2:1, v/v)
Elution 2: fraction of free fatty acids are eluted with 3 mL diethyl ether – acetic acid (98:2, v/v)
Elution 3: fraction of polar lipids are eluted with 4 mL methanol
Evaporation: stream of nitrogen
Authors: Püttmann et al.
Source: CLIN. CHEM. 39/5, 825–832 (1993)